Additional file 1 of Boosting cisplatin chemotherapy by nanomotor-enhanced tumor penetration and DNA adducts formation

Additional file 1: Figure S1. DLS analysis of F68@TA prepared by different mass ratio of F68 and TA. (n=3). Figure S2. TEM images of F68@TA@Ag NPs prepared with different content of AgNO3. Figure S3. (A) Size distributions and (B) zeta potentials of F68@TA, F68@TA/CDDP, and AINR solutions. Figure S4. Tyndall Effects of F68@TA/CDDP solution at different pHs for 1 h. Figure S5. DLS analysis of F68@TA/CDDP treated with PBS at different pHs for 48 h. Figure S6. HPLC chromatograms of different solvents and corresponsive CDDP (40 μg/mL) solution for 2 d. Figure S7. (A) Graphs and (B) UV-vis spectrum of F68@TA@Ag solutions with different concentration of H2O2. Figure S8. TBE-PAGE gel electrophoregram of DNA. 1: unmethylated single strand DNA; 2: unmethylated single strand DNA cut by restriction enzyme DnpII; 3: m1A methylated DNA; 4: m1A DNA sheared by DnpII. Figure S9. A) The tracking paths of F68@TA@Ag over 20 s in 0, 2.5, 5, 10, 25 and 50 mM H2O2. B) Average MSD versus time interval (Δt) analyzed from tracking trajectories. C) Corresponding diffusion coefficient values of F68@TA@Ag at various concentration of H2O2. (n=30). Figure S10. Fluorescence emission spectrums of FITC and RB dissolved in PBS at different pHs. Figure S11. A) Flow cytometry analysis and B) Fluorescence semi-quantitative analysis of 4T1 cells treated with F68@TA/RB for different time. Figure S12. A) CLSM images and B) Fluorescence semi-quantitative analysis of 4T1 cancer cells after treatments with F68@TA/RB (RB: 10 μg/mL) for different time. Scale bar: 25 μm. Figure S13. Fluorescence semi-quantitative analysis of H2O2 in 4T1 cells after treatment with different concentration of CDDP. Figure S14. Fluorescence semi-quantitative analysis of Cl- in 4T1 cells after treatment with CDDP, F68@TA, F68@TA@Ag and AINR. Figure S15. Fluorescence semi-quantitative analysis of Fe2+ in 4T1 cells after treatment with CDDP, F68@TA, F68@TA@Ag and AINR. Figure S16. Flow cytometry analysis of the fluorescence intensity in A) Hs578Bst cells and B) 4T1 cells treated with F68@TA/RB (RB: 5 μg/mL) for different time. C) Fluorescence semi-quantitative analysis of 4T1 cells and Hs578Bst cells. Figure S17. Fluorescence semi-quantitative analysis of Cl- in tumor tissues. Figure S18. Fluorescence semi-quantitative analysis of Fe2+ in tumor tissues. Figure S19. Body weight changes of different groups. (n = 5). Figure S20. H&E staining of tissues exfoliated from different groups. Figure S21. Serum biochemical index. A) Liver function and B) Kidney function index of mice treated with different formulation. 1: Control, 2: F68@TA, 3: F68@TA@Ag, 4: CDDP, 5: F68@TA/CDDP, 6: AINR, and concentration of CDDP was set as 1 μg/mL. Figure S22. The changes of tumor volume of 4T1 cell bearing mice with the treatment of AINR (CDDP: 2 mg/kg), 2 mg/kg CDDP, and 3 mg/kg CDDP. (n=5). Table S1. The DNA sequences of unmethylated DNA and methylated DNA