Additional file 1 of Natural lipid nanoparticles extracted from Morus nigra L. leaves for targeted treatment of hepatocellular carcinoma via the oral route

Supplementary Material 1: Figure S1. Percentages and MFIs of DiO-labeled MLNPs internalized by L929 cells after co-incubation for 1, 3, and 5 h, respectively. Each point represents the mean ± S.E.M. (n = 3; *p < 0.05, **p < 0.01, and ***p < 0.001). Figure S2. Hemolysis test. (a) Digital photo and (b) hemolysis rates of erythrocytes with the treatment of Triton X-100 (positive control; 0.1%, w/v), PBS (negative control), and MLNPs (various protein concentrations). Each point represents the mean ± S.E.M. (n = 3). Figure S3. (a) Body weight and (b) organ index of different mouse groups. The amounts of (c) ALT, (d) AST, (e) TBIL, (f) GGT, (g) CRE, and (h) BUN in the serum from different mouse groups. Each point represents the mean ± S.E.M. (n = 3; *p < 0.05). Figure S4. Blood test results from mice with different treatments. Each point represents the mean ± S.E.M. (n = 3; *p < 0.05). Figure S5. Histological analysis of the five principle organs (heart, liver, spleen, lung, and kidney) from the mice with the treatment of MLNPs via i.v. and oral routes (Scale bar = 200 µm, n = 3). Figure S6. (a) The organ index and (b) histological analysis of the four principle organs (heart, spleen, lung, and kidney) from mice with different treatments (Scale bar = 200 µm). Each point represents the mean ± S.E.M. (n = 5