Deletion and complementation of <i>Bcgbl1</i> in <i>B</i>. <i>cinerea</i>.

(A) Schematic diagram showing the strategy to delete Bcgbl1. (B) A schematic diagram showing the construct for complementation of Bcgbl1. (C) PCR confirmation of deletion of Bcgbl1 in different mutants. (D) Southern blot confirmation of deletion of Bcgbl1. The DNA probe position was shown in A. (E) RT-qPCR confirmation of deletion and complementation of Bcgbl1 in different mutants. (TIF)