Isolation of gene-deletion mutants.
- Masayuki Nakamura (2537542)
- Yuino Yasukawa (5617325)
- Akira Furusawa (4353106)
- Tamao Fuchiwaki (5617328)
- Takashi Honda (775852)
- Yuta Okamura (5617331)
- Kiyotaka Fujita (5617334)
- Hisashi Iwai (5617337)
Publication date
August 2018
DOIAbstract
<p>(A) Genetic map of <i>xehypBA1</i>, <i>xehypBA2</i>, and <i>xehypAA</i> of <i>X</i>. <i>euvesicatoria</i>. <i>Solid lines</i> below the map represent fragments used to construct mutants. <i>Dashed lines</i> indicate the deletion regions. <i>Arrows</i> indicate primers used to confirm the gene deletion. PIP box, plant-inducible promoter box. (B) Confirmation of gene-deletion mutants by PCR. M, DNA molecular weight marker; WT, wild type; ΔBA1, Δ<i>xehypBA1</i>; ΔBA2, Δ<i>xehypBA2</i>; ΔBA-AA, Δ<i>xehypBA1-BA2-AA</i> (constructed from Δ<i>xehypBA1</i>).</p
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