Supplemental Figure 1

Verification of the cpnC- mutant cell line using PCR and genome sequencing. A) Diagram indicating where PCR primers anneal within the cpnC gene (black) and bsr gene (red). Primers 1 and 2 amplify a 660 bp fragment of the cpnC gene coding region. Primers 1 and 3 amplify a 783 bp fragment if homologous recombination has occurred to replace most of the cpnC gene with the bsr gene. B) The above primers were used in PCR with genomic DNA isolated from both parental and cpnC- cells and PCR samples were analyzed by agarose gel electrophoresis. No genomic DNA (no DNA) was used in PCR as a negative control. C) Whole genome sequencing and analysis was performed on the cpnC- mutant and parental cell lines. Sequence reads were mapped to a reference genome and shown in Interactive Genome Viewer. Chimeric paired reads in which one member maps to the pPLBLP plasmid and the other maps to the reference are also shown.