Mutations from the calli flow to the regenerated plants.

<p>The genotype and detailed sequence variation of some typical mutants from chimera, homozygote and heterozygote are presented. Each dashed line represents a deleted nucleotide. The nucleotide in gray represents substitution. WT, wild-type sequence; d, base deletion; s, base substitution. The number after ‘d’ or ‘s’ represents the number of bases that have been deleted or substituted. The box marks the variant sequence which might result from mismatch repair in <i>E</i>. <i>coli</i> during PCR product cloning.