Dasatinib and ponatinib induce expression of leukocyte adhesion molecules on HUVECs.

A. Representative western blots for VCAM1, ICAM1, P-Selectin, and E-Selectin of HUVECs treated for 24 hours with the indicated TKIs. B-E. Quantification of VCAM1 (B), ICAM1 (C), P-Selectin (D), and E-Selectin (E) relative to GAPDH after treatment with TKI or DMSO control. Data are presented as mean ± SEM, with N = 5 independent experiments per treatment. Statistical analysis by one way ANOVA with Dunnett’s multiple comparisons test with comparison of each mean to DMSO. *p<0.05, **p<0.01, ***p<0.001.