Dasatinib induces changes to the actin cytoskeleton and cell-cell junctions in human engineered microvessels (hEMVs).

A. Representative images of hEMVs stained for VE-Cadherin (top row) or Actin (bottom row) after treatment with indicated BCR-ABL TKI or vehicle control for 3 hours. Scale bar = 150 μM. B. Quantification of VE-Cadherin staining for hEMVs treated with each TKI or DMSO control. C. Quantification of the ratio of junctional to cytoplasmic actin. Statistical analysis by one way ANOVA with Dunnett’s multiple comparisons test with comparison of each mean to DMSO. *, p <0.05, *** p < 0.001 **** p < 0.0001.