Dasatinib and ponatinib act on HUVECs and leukocytes to enhance leukocyte-endothelial cell adhesion.

A. Representative images of U937 cells adherent to HUVECs after pre-treatment of HUVECs alone (top row), U937 cells alone (middle row), or pre-treatment of both cell types (bottom row). Scale Bar = 200 μM. B. Quantification of the number of adherent U937 cells relative to DMSO control after HUVEC pre-treatment with TKI. C. Quantification of adherent U937 cell relative to DMSO control after U937 leukocytes were pre-treated with TKI. D. Quantification of adherent U937 cells relative to DMSO control after both U937 cells and HUVECs were pre-treated with each TKI. Data are presented as mean ± SEM, with N = 3–4 independent experiments per treatment. Statistical analysis by one way ANOVA with Dunnett’s multiple comparisons test with comparison of each mean to DMSO. **p<0.01, ***p<0.001, ****p<0.0001.