Analysis of Kkv trafficking [Dataset]

All images are single confocal sections except A-C, which are projections of confocal sections. (A, A’) In trachea of wild-type embryos, Kkv is present in the apical region (blue arrows) and in intracellular vesicles (yellow arrowheads). (B) In kkv mutants unable to polymerise chitin, Kkv is not properly localised. (C) GFP-Kkv localises to the apical region (blue arrows) and in intracellular vesicles (yellow arrowheads). (D) When reb and GFP-kkv are coexpressed in salivary glands, Kkv is present in the apical membrane (blue arrow), in intracellular vesicles (yellow arrowhead), and also in punctae in the lumen (pink arrowheads). This is clearly observed in orthogonal sections (D’). (E) These luminal punctae corresponded to membranous structures. (F, F’) In contrast, when GFP-kkv is expressed alone, luminal punctae are absent, and Kkv is only found apically (blue arrow) and in intracellular vesicles (yellow arrowhead). (G) Luminal punctae (pink arrowheads) are also observed in the trachea of embryos overexpressing reb and GFP-kkv. (H) When endocytosis is prevented, the coexpression of reb and GFP-kkv in salivary glands still leads to formation of Kkv luminal punctae (pink arrowheads). (I) Quantifications of the number of intracellular Kkv vesicles in salivary glands when expressing GFP-kkv (I’), reb and GFP-kkv (I”), and GFP-kkvΔCC. n is the number of salivary glands analysed. The underlying data for quantifications can be found in the S1 Data. Scale bars: 10 μm.Peer reviewe