Analysis of Kkv apical distribution in salivary glands and Co-IP.

(A, B) Kkv distribution on the apical surface of a control (A) and an embryo expressing reb in salivary glands (fkhGal4>UAS-reb, B) and zoomed images for Kkv punctae (magenta) on the apical cell area marked by arm (green) of a control (C) and a fkhGal4>UAS-reb (D) embryo. The corresponding observed F function for the control (E) and the fkhGal4>UAS-reb (F) are displayed within and below the reference simulated random distributions (black) and the 95% confidence interval (light gray), respectively, indicating a random spatial pattern for the control and a tendency towards the formation of aggregates for the reb ectopic expression. (G) SDI histogram for the F-Function of the control (blue) and the fkhGal4>UAS-reb (magenta) samples. A significant difference between the frequency distributions for each group of individuals has been observed. (Kolmogorov–Smirnov D = 0.6667, p H) Frequency distribution histograms for the Nearest Neighbour Distances (NNDs) between Kkv punctae in control (blue) and fkhGal4>UAS-reb samples. The distribution of values between the two groups is found significantly different (Kolmogorov–Smirnov D = 0.1463, p S1 Data. (J, K) Western blot using αKkv (J, two different exposure times are shown) or αReb (K) of embryo extracts that were subjected to immunoprecipitation with αKkv or an unrelated antibody (mock). Input correspond to 7.5% of the immunoprecipitated material. The position of MW markers (in kDa) is indicated. Scale bars A, B: 5 μm; C, D: 2 μm. (TIF)