S4 Fig -

(A) Projection of confocal sections of salivary glands shows that Kkv is normally expressed in salivary glands and accumulates apically. (B-D) Single confocal sections of trachea at early stages. Single chitin punctae do not colocalise with deacetylases or Gasp. (E, F) Single confocal sections of salivary glands expressing serp and verm. Single chitin punctae do not colocalise with deacetylases. (G) Frames from live imaging movie 2 show that common GFP-Kkv and chitin punctae (yellow arrow) can separate from each other; many GFP-Kkv (green arrow) and chitin puncta (red arrow) do not colocalise. (H) The luminal GFP-Kkv punctae are labelled by GFP and Kkv. Scale bars A, E, F: 5 μm; C-D, G: 10 μm. (TIF)