The effect of the combination of thiotriazoline and corvitin on the values of some parameters of the cellular and humoral immune system during the experimental reproduction of allergic alveolitis under immobilization stress

Galii-Lutska V. V. The effect of the combination of thiotriazoline and corvitin on the values of some parameters of the cellular and humoral immune system during the experimental reproduction of allergic alveolitis under immobilization stress. Journal of Education, Health and Sport. 2022;12(10): 373-380. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.10.043 https://apcz.umk.pl/JEHS/article/view/40828 https://zenodo.org/record/7311844 The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 1, 2021. No. 32343. Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences). Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 1 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159. Przypisane dyscypliny naukowe:Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu). © The Authors 2022; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 03.10.2022. Revised: 12.10.2022. Accepted: .31.10.2022. UDC: 616.312-2-002-616.314.17-008.6):612.014.484:610. – 092.7 The effect of the combination of thiotriazoline and corvitin on the values of some parameters of the cellular and humoral immune system during the experimental reproduction of allergic alveolitis under immobilization stress V. V. Galii-Lutska Danylo Halytsky Lviv National Medical University, Lviv, Ukraine Abstract The purpose of the study was to determine the effectiveness of the combination of corvitin and thiotriazoline for the correction of levels of T- and B-lymphocytes, circulating immune complexes (CIC) of the blood of male guinea pigs (GP) if experimental allergic alveolitis (EAA) and immobilization stress (IS) are combined. The study was performed on 50 male GP weighing 180-210 g. EAA was reproduced by the method of O.O. Orekhov, Yu.A. Kyrylov. The production of IS was carried out by the method of P.D. Horizontov. The content of T- and B-lymphocytes in the blood was determined by the method of E.F. Chernushenko, L.S. Kogosova. CIC were detected by the method of Haskova V., Kaslik J., Math J., Matejckava M. The withdrawal from the experiment was carried out by the method of decapitation after previous administration of nalbuphine hydrochloride in the experimental group (EG) of intact GP; in the EG of EAA and IS during the study days corresponding to the stages of development of IS: 1st – anxiety, 14th – resistance, 24th – exhaustion; EG of EAA with IS on the 24th day after pharmacocorrection with the above-mentioned combination of drugs. There was a progressive decrease in the level of T-lymphocytes by 33,47% (p<0,01), 36,23% (p<0,01) and 39,62% (p<0,01), an increase in B-lymphocytes by 27,82% (p<0,01), 131,13% (p<0,01) and 44,37% (p<0,01), an increase in CIC by 41,15 % (p<0,01), 46,62% (p<0,01), and 50,78% (p<0,01) on the 1st, 14th and 24th days respectively, compared to the indicators of the control group under the combination of EAA and IS. An increase in T-lymphocytes by 40% (p<0,01), a decrease in B-lymphocytes by 24,31% (p<0,01), and a decrease in the level of CIC by 26,08% (p