Supplementary data

Figure S1: High resolution respirometry protocol for permeabilized fibers as analyzed in DatLab. The blue line represents the oxygen concentration, and the red line represents the oxygen flux rate. The vertical lines indicate substrate titrations, with the substrate or substrate combination written underneath.Figure S2: High resolution Respirometry protocol for assessment of P/O ratio, as analyzed in DatLab. The blue line represents the oxygen concentration, and the red line represents the oxygen flux rate. The vertical lines indicate substrate titrations, with the substrate or substrate combination written underneath. The black circles represent the regions of oxygen concentration that are marked to calculate the P/O ratio, where region 1 represents the oxygen concentration before the addition of ADP, region 2 represents the decline in oxygen during oxidative phosphorylation, and region 3 represents the oxygen concentration when the process of oxidative phosphorylation has ended.Table 1. Mean CV (%) of respiration measurements between chambers in the same oxygraph for the same sample in permeabilized fibers at different temperature and pH settings. The following protocol steps are included: State 2 (pyruvate + malate), state 4o (pyruvate + malate + ADP + oligomycin), CI respiration (pyruvate + malate + ADP) and CI+II respiration (pyruvate + malate + ADP + succinate).