MOESM2 of Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate

Additional file 2: Figure S2. Culture of E. coli BL21 pRhotHi YFP under oxygen-limited conditions in synthetic Wilms-MOPS containing 20 g L−1 glucose and 0.2 M MOPS buffer with a filling volume (VL) of 1100 µL. The dissolved oxygen tension (DOT) was monitored by means of dispersed infrared oxygen-sensitive nanoparticles. pH, optical density (OD600), glucose, acetate and lactate concentrations were evaluated from offline analysis of parallel wells. Cultivation conditions: synthetic Wilms-MOPS medium with 20 g L−1 glucose and 0.2 M MOPS buffer; 48 round deep-well MTP without optodes, OD600,start = 0.05, n = 1000 rpm, shaking diameter d0 = 3 mm, 37 °C