Oxygraphic example output of protocols with intact and permeabilized INS-1 832/13 cells (at normoxia).

<p>(A) Protocol with intact cells, (B) SUIT_CI protocol with permeabilized cells with glutamate as only reducing substrate, (C) SUIT_CII protocol with permeabilized cells with succinate as only reducing substrate, <b>(</b>D) SUIT_CI+II protocol with glutamate and succinate as reducing substrates. The blue line represents the oxygen concentration (nmol O₂/mL) in the experimental chamber. The red line represents oxygen flux (pmol O₂/s/10⁶ cells), the negative time derivate calculated from the measured oxygen concentration, normalized to the number of cells. ROUTINE: ROUTINE respiratory state of basal respiration, LEAK: LEAK respiratory state of uncoupled respiration, OXPHOS: OXPHOS respiratory state of maximum phosphorylative capacity, ETS: ETS respiratory state of the maximum capacity of the ETS, ROX: ROX respiratory state of residual oxygen consumption, Dig: digitonin, G: glutamate, D: ADP, S: succinate, c: cytochrome <i>c</i>, F: FCCP, Rot: rotenone, Ama: antimycin A.