Respirometry data at room temperature (23˚C).

<p>Rat lung mitochondria were incubated in 0.55 ml buffer containing 2 mM Pi, and 0.55 mg mitochondria protein, buffer pH = 7.2. Average oxygen concentrations in buffer are shown under state 2, state 3, state 4 and uncoupled state 3 using pyruvate (PYR) + malate (MAL) (n = 4, panel A), MAL + glutamate (GLU) (n = 1, panel B), or succinate (SUC) (n = 4, panel C) as substrates. ADP and FCCP was added to buffer at 3.5 min and 6.5 min, respectively. Symbols are experimental data (mean ± SE) and solid lines are integrated model fits to the data. For the integrated model fits to the uncoupled state 3 data, the estimated value of the proton leak parameter <i>T</i>_{<i>maxf</i>, <i>LEAK</i>} in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197921#pone.0197921.t002" target="_blank">Table 2</a> was increased by seven folds. Panels D, E, and F show average oxygen consumption rates (OCR, nmol/min/mg) under states 2, 3 and 4 using PYR + MAL (n = 4), MAL + GLU (n = 1), or SUC (n = 4) as substrates. Blue bars are experimental data (mean ± SE) and red bars are integrated model fits to the data.