Figure 4 from β-Catenin Drives Butyrophilin-like Molecule Loss and γδ T-cell Exclusion in Colon Cancer

Activation of β-catenin decreases butyrophilin-like molecule expression. A and B, Correlation between indicated genes as determined by TempO-seq and γδ T-cell density determined by IHC in the Scotland cohort. Units on axes are normalized read counts × 103. Each dot represents one tumor (n = 77 left and 82 right). P and r values determined by Pearson correlation. C, Correlation between CTNNB1 or SOX9 expression and BTNL3 or BTNL8 expression in the Marisa cohort (28). Units on axes are normalized counts × 103. Each dot represents one tumor (n = 258). P and r values determined by Pearson correlation. D, Images of organoids derived from indicated mouse models taken 4 days after tamoxifen treatment. E, Fold change in expression levels of indicated genes in organoids from various genotypes measured at indicated days after tamoxifen treatment. Each dot represents one organoid derived from one mouse (n = 3). F, Fold change in expression levels of indicated genes in WT organoids treated with 3 or 10 μmol/L CHIR-99021 for indicated days. Each dot represents one organoid derived from one mouse (n = 3). Data are presented as mean ± SD. *, P P P post hoc test).