Figure 3 from β-Catenin Drives Butyrophilin-like Molecule Loss and γδ T-cell Exclusion in Colon Cancer

Expression of butyrophilin-like molecules is reduced in gut tumors. A, Images of intestinal tissue from indicated models (n = 4) stained for Btnl1 mRNA. T, tumor; scale bar, 100 μm. B, Images of intestinal tissue from indicated models (n = 4) stained for Btnl1 mRNA; scale bar, 100 μm. C, Butyrophilin-like mRNA expression shown by bar graph generated from RNA-seq data from WT (n = 9), VAF/F (n = 36), and VAF/FK (n = 17) mice. Data are presented as mean ± SD. D, Expressions of BTNL3 and BTNL8 in normal human colonic tissue and tumor tissue from TCGA (n = 19 normal, 101 tumor; ref. 9) and Skrypczak (n = 24 normal, 45 tumor; ref. 27) datasets. Data are presented as median ± min/max. E–G, Correlation between indicated molecules as determined by TempO-Seq and γδ T-cell density determined by IHC in the Scotland cohort from 77 matched pairs. Units on axes are normalized read counts x 103. Each dot represents one tumor. P and r values determined by Pearson correlation. *, P P P U test or one-way ANOVA followed by Tukey post hoc test).