Overview of actinomycin D treatment on transcript counts compared to iron-induced reduction of mRNA transcripts.

(A) Changes in transcript counts over time (6 h) in iron-recovery (rec; orange), actD-treated cells (actD–red, actD-Fe–grey). All samples shown here were low-iron samples. ActD cultures received actinomycin D and actD-Fe cultures received actinomycin D and iron. Rec are low-iron samples that received only iron. (B) Table of half-lives for transcripts analyzed in the ST-experiment (in min). Values with an asterisk are excluded from the average. (C) Bar graphs of half-lives of the 7 rapidly declining iron-responsive genes. Transcripts with an asterisk revealed significant differences between actD and iron-recovery samples. (D) Bar graphs of half-lives for non-iron-responsive genes.