Transcript counts of potential housekeeping genes.

(A) Transcript counts of potential housekeeping genes, including the total amount of transcript reads over all transcripts (sum). The significant difference between low- and high-iron samples in the 24_probeset for the nuclear import-export protein is based on loading issues. The sum of all transcripts over all genes (sum) reveals the same trend indicating that the loading amounts were higher than expected. (B) Box plots representing transcript levels of high-. low-iron, and iron-recovery samples are plotted with Student’s t-test used for significance analysis. (C) Box plots of transcript counts of daytime and nighttime samples. These samples are further divided into low-iron, high-iron, and recovery samples from the 24_probeset NanoString analysis. Daytime boxplots are shown in orange, and nighttime boxplots are presented in blue. (D) Box plots of transcript counts of daytime and nighttime samples. The samples are further divided into low-iron, high-iron, recovery samples from the 48_probeset NanoString analysis. Day time boxplots are shown in orange and nighttime samples are in blue. Overall, the top row shows results from DNAj, the second row shows the nuclear import-export protein, and the third row is a hypothetical transporter protein. The sum of all genes is the bottom row. High iron samples are shown in black, low iron samples are shown in blue and recovery samples are shown in orange. Statistically significant P values are indicated as * (TIF)