Ribosomal density profile for highly translated genes at single nucleotide resolution.

<p><b>A</b>. The first <i>600</i> nts; all genes are aligned to the ORF's 5′ end. <b>B</b>. The last <i>600</i> nts; all genes are aligned to the ORF's 3′ end. The y-axes represent the mean RD in logarithmic scale at specific locations along the ORF; the x-axes represent the location of a nucleotide measured as the distance from the ORF's 5′ end (positive numbers at <b>A</b>.) or distance from the ORF's 3′ end (negative number at <b>B</b>.). The red line describes the <i>20%</i> of genes with the longest mRNA half-life; the green line describes the <i>20%</i> of genes with the shortest mRNA half-life, and the blue line describes all the genes. The inset in each plot includes the RD median for each group of genes, from left to right: the <i>20%</i> of the genes with the shortest half-life, all genes, and the <i>20%</i> of the genes with the longest half-life. The number above the arrow is the P-value corresponding to the Wilcoxon rank sum test between the local averaged RD of genes with the <i>20%</i> longest and shortest half-life.