AR-mediated destabilization of cyclin D1 mRNA in HPr-1AR.

<p>(A) Experimental design scheme depicts transcriptional inhibition by actinomycin D (ActD), DHT treatment, and mRNA isolation. Cells were treated with transcription inhibitor, ActD, for 1 hour prior to treatment with 10 nM DHT or vehicle control, and total RNA was harvested at the indicated time points for quantification by QPCR. (B) Transcription of the PYGO2 control gene was unchanged by androgen, and the half-life of its mRNAs was unaffected. The half-lives of cyclin D2, CDK4, and CDK6 mRNAs were unchanged by DHT treatment compared to vehicle control, whereas the cyclin D1 mRNA half-life was 5 hours (h) in DHT-treated samples compared to 10 hours in control samples. Data represent the mean ± SEM, n = 3.