Four example genes were chosen to demonstrate the range of half-lives that can be measured in this experiment. The black dots represent data points from each of the microarray replicates for that time point, including the 0 time point with and without actinomycin D treatment. The colored lines represent the fitted decay curve. The half-life (t) for each example is listed.<p><b>Copyright information:</b></p><p>Taken from "Whole-genome analysis of mRNA decay in reveals a global lengthening of mRNA half-life during the intra-erythrocytic development cycle"</p><p>http://genomebiology.com/2007/8/7/R134</p><p>Genome Biology 2007;8(7):R134-R134.</p><p>Published online 7 Aug 2007</p><p>PMCID:PMC2323219.</p><p></p
Degradation of mRNA is one of the key processes that control the steady-state level of gene expressi...
Experimental studies on mRNA stability have established several, qualitatively distinct decay patter...
A general program was written which simulates radioactive labeling of RNA . The program was used to ...
Four separate time course experiments were conducted at 12-hour intervals using a single source cult...
<p>Relative mRNA number as a function of the time after the interruption of transcription. (A) Two...
<p>Half-life of mRNA at each time point was determined by real-time RT-PCR. *decay rate was too low ...
<p>Actinomycin D (Act D) mRNA decay of B<sub>1</sub>R WT and SV transcripts in H2126 measured at 0, ...
<p>(A) Western blot analysis for VNP/GFP, Nanog, Oct4and a-Tub proteins in Nd, E14tg2a and TNG-A ES ...
Although mRNA decay rates are a key determinant of the steady-state concentration for any given mRNA...
<p>A. Distribution of genome-wide decay profiles across the timecourse experiment (x-axis), where ea...
<p>Half-life distributions of the genes from the bottom <i>20%</i> RD (green curve), top <i>20%</i> ...
<p>The patterns are obtained from a systematic fitting procedure applying Eqs. (1) and (9) or (10) t...
<p>For each group, the histogram shows the mean value ± standard error of the half-lives of mRNAs -e...
<p>(A-C), Differential decay in three representative <i>E</i>. <i>coli</i> operons, depicted by norm...
Background: Gene expression is the result of the balance between transcription and degradation. Rece...
Degradation of mRNA is one of the key processes that control the steady-state level of gene expressi...
Experimental studies on mRNA stability have established several, qualitatively distinct decay patter...
A general program was written which simulates radioactive labeling of RNA . The program was used to ...
Four separate time course experiments were conducted at 12-hour intervals using a single source cult...
<p>Relative mRNA number as a function of the time after the interruption of transcription. (A) Two...
<p>Half-life of mRNA at each time point was determined by real-time RT-PCR. *decay rate was too low ...
<p>Actinomycin D (Act D) mRNA decay of B<sub>1</sub>R WT and SV transcripts in H2126 measured at 0, ...
<p>(A) Western blot analysis for VNP/GFP, Nanog, Oct4and a-Tub proteins in Nd, E14tg2a and TNG-A ES ...
Although mRNA decay rates are a key determinant of the steady-state concentration for any given mRNA...
<p>A. Distribution of genome-wide decay profiles across the timecourse experiment (x-axis), where ea...
<p>Half-life distributions of the genes from the bottom <i>20%</i> RD (green curve), top <i>20%</i> ...
<p>The patterns are obtained from a systematic fitting procedure applying Eqs. (1) and (9) or (10) t...
<p>For each group, the histogram shows the mean value ± standard error of the half-lives of mRNAs -e...
<p>(A-C), Differential decay in three representative <i>E</i>. <i>coli</i> operons, depicted by norm...
Background: Gene expression is the result of the balance between transcription and degradation. Rece...
Degradation of mRNA is one of the key processes that control the steady-state level of gene expressi...
Experimental studies on mRNA stability have established several, qualitatively distinct decay patter...
A general program was written which simulates radioactive labeling of RNA . The program was used to ...