S1 Fig -

A) A schematic of the construct used to generate lkyKO flies. B) Validation of the KO genotype by PCR. C-F) IF staining for acTub and Vasa of germaria of the indicated genotypes. “double KO” = lkyKO/lkyKO;; dTATKO/dTATKO. G) A representative image of a tip of an ovariole of wild type control (yw) and Double KO showing remaining acTub in later stages of germline and FCs. Flies were used at 0–7 days post-eclosion. Scale Bars; 10μm. Flies were used at 0–7 days post-eclosion. Scale Bars; 10μm. (TIF)