⍺-K40-acetylation by Lky is required for maintenance of oogenesis during fly aging.

A-F) Representative images for comparison of wildtype (yw, A, C, E) and lkyKO (lkyKO/Df, B, D, F) ovarioles, showing the egg chamber fusion phenotype in lkyKO. White arrowheads in C-F indicate oocytes (Orb, green) in indicated stages of egg chambers of wildtype (C, E) and lkyKO (D, F). G) A graph shows frequency of ovarioles with egg chamber fusion, scored at 7, 14, and 21 days post-eclosion for the indicated genotypes. H) A graph shows frequency of ovarioles with egg chamber fusion in lkyKO with or without germline (nosGal4 driven)- or somatic cell (tjGal4 driven)- specific expression of lky. I) A graph shows frequency of ovarioles with egg chamber fusion in ovaries of lky RNAi induced under the germline (nosGal4)- or somatic cell (tjGal4) drivers. J) A graph shows frequency of ovarioles with egg chamber fusion after germline (nosGal4 driven)- or somatic cell (tjGal4 driven)- specific expression of αTubulinK40Q or αTubulinK40R transgenes. Flies were used at day 21 post-eclosion for H-J. K-L) Representative images of αTub84BK40A homozygous knock-in ovarioles showing egg chamber fusion at indicated stages. Flies were used at day 21 post-eclosion. M) A graph shows frequency of ovarioles with egg chamber fusion in αTub84BK40A knock-in ovaries at indicated ages. Heterozygous flies (αTub84BK40A/+) were used for the control. Adjusted P values from Šidák’s multiple comparisons test are shown for G, H, and J, and Dunnett’s multiple comparisons test for I. For all data points, a minimum of 20 ovaries were used from two individual crosses. Scale Bars: 10 μm.