Vasa protein is often detected in FCs in <i>lky</i>^<i>KO</i> ovaries.

A, B) Representative images of IF staining of germaria. lkyKO/Df image shows abnormal localization of Vasa in the FC layer (white arrowheads). C-F) IF staining of Stage3-4 egg chambers for Vasa and TJ, a FC marker, showing Vasa protein in TJ-positive FCs (white arrowhead) in lkyKO. C and D shows surface views of an egg chamber, E and F show cross sections. For A to F, flies were used at days 7–14 post-eclosion. G) A frequency of ovarioles with any ectopic Vasa localization detected in FCs, comparing control versus lkyKO for 7, 14, and 21 days. Percentages of ovarioles containing any FCs which stain positive for Vasa (Vasa in FC cytoplasm) were manually counted. H) A graph shows frequency of ovarioles containing Vasa-positive FCs in indicated genotypes. Germline-specific expression of lky in the germline (under the nosGal4 driver) reduced the frequency, but somatic cell-specific expression of lky (under the tjGal4 driver) had no effect. I, J) Representative surface views of Vasa-positive FCs in heterozygous control (I) or homozygous (J) of αTub84BK40A knock-in egg chambers (Stage3-4). K) A graph shows frequency of ovarioles containing any Vasa-positive FCs in αTub84BK40A heterozygous (control) or homozygous (K40A) at days 7, 14, and 21 post-eclosion. Adjusted P values from Šidák’s multiple comparisons test are shown for G, H, and K. For all data points, a minimum of 20 ovaries were used from two individual crosses. Scale Bars: 10 μm.