Investigation of wing growth hit <i>CG11103</i> using CRISPR/Cas9 gene disruption.

(A) Schematic of the genomic locus of candidate CG11103, position of the CRISPR target site and the mutant allele analysed. Flies carrying an in-frame mutation were used as control. (B) Gene tree for TM2 domain proteins in humans and Drosophila, with an archaeal TM2 protein as an outlier. Tree built using sequence of TM2 domains alone using T-Coffee. A fourth TM2 domain protein is present in Drosophila and humans (Wurst/DNAJC22) which has additional TMDs and a DNAJ domain and appears to play a role in clathrin-mediated endocytosis [116]. (C–E) Cuticle phenotypes of embryos laid by control females and mutant females (homozygous or over a deficiency). (F, G) Micrographs of embryos laid by control females and homozygous mutant females stained against the pan-neuronal marker Elav. Scale bars: 50 μm. (H) Schematic of the genomic locus of CG10795, position of CRISPR target sites and the alleles analysed. Flies without an indel were used as control (CG10795_4). (I, J) Cuticle phenotypes of embryos laid by control or mutant females. (K, L) Micrographs of embryos laid by control or mutant females stained for the pan-neuronal marker Elav. Scale bars: 50 μm.