SDS-PAGE and Western blot analysis of PfGCS1-SAPN.

(A) SDS-PAGE analysis of PfGCS1-SAPN expression. Lanes 1–4: E. coli BL21(DE3)-pET24a, lanes 5–9: E. coli BL21(DE3)-PfGCS1-SAPN-pET24a. Lanes 1, 5: Before induction; lanes 2 and 6: 1h after induction with IPTG; lanes 3 and 7: 2h after induction; lanes 4 and 8: 4h after induction; lane 9: 16h after induction; and lane 10: molecular weight protein marker (Fermentas, 116–14.4 kDa). (B) SDS-PAGE analysis of purified PfGCS1-SAPN. Lanes 1–4: purified PfGCS1-SAPN. Lane 5: molecular weight protein marker (Fermentas, 116–14.4 kDa). (C) Western blot analysis of PfGCS1-SAPN protein with anti-His tag mAb. Lanes 1 and 2: Before induction and 4h after induction of E. coli BL21(DE3)-pET24a as negative controls, respectively. Lanes 3 and 4: Before induction and 4h after induction of E. coli BL21(DE3)- PfGCS1-SAPN-pET24a, respectively. Lane 5: purified PfGCS1-SAPN. Lane 6: molecular weight protein marker (Fermentas, 116–14.4 kDa).