Solubilization and purification of PfHep1.

<p>(<b>A</b>) SDS-PAGE (10%) analysis of the solubility of PfHep1 before and after addition of sarcosyl. Lane M: protein markers in kDa, lanes 1–2: supernatant and pellet fractions of cells not treated with sarcosyl, lanes 3–4: supernatant and pellet fraction of cells treated with 3% sarcosyl. (<b>B</b>) Western analysis using anti-His antibody. (<b>C</b>) Purification of PfHep1 after solubilization with 3% sarcosyl, lane M: protein markers in kDa, lane 1: <i>E</i>. <i>coli</i> M15 ([pREP4]; pQE30-PfHep1) 4 hrs post IPTG induction, lane 2: fraction unbound to the cOmplete His-tag purification resin, lanes 3–5: washes using 50 mM imidazole, lanes 6–8: elutions of PfHep1 using 750 mM imidazole, and lane 9: bead fraction. (<b>D</b>) Western analysis for detection of PfHep1 using anti-His antibodies.