<p>Lane M, marker; lane 1, the soluble fraction of the <i>E. coli</i> cell lysate containing TIM; lane 2, purified TIM; lane 3, the soluble fraction of the <i>E. coli</i> cell lysate containing; lane 4, purified ALD; lane 5, the soluble fraction of the <i>E. coli</i> cell lysate containing FBP; lane 6, purified FBP; lane 7, the soluble fraction of the <i>E. coli</i> cell lysate containing PGI; and lane 8, purified PGI.</p
(Lane 1—Protein Standard (NEB, USA), Lane 2—Negative Control (E. coli BL21DE3), Lane 3—Uninduced clo...
<p>The purified proteins were separated on a 12.5% gel under reducing or non-reducing conditions: (A...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...
<p>Protein weight marker (lane M), cell extract of <i>E. coli</i> LMG194/pBADRSFP (lane A), pooled f...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<div><p><b>coli transformants</b>. </p> <p>Lane 1, <i>E</i>. <i>coli</i>/pET-MS-L-G; Lane 2, <i>E</i...
<p>Lane 1, Protein Molecular Weight Marker (Fermentas); lane 2, soluble <i>E. coli</i> BL21 (DE3) [p...
<p>M: molecular weight markers; 1: lysates of pre-IPTG-induced <i>E</i>. <i>coli</i> in 20 μL of cel...
<p>(A) Distribution of PD in induced bacteria after large-scale expression. Lane M, pre-stained prot...
<p>A) Lane M, protein molecular weight markers; lane 1, uninduced cell sample; line 2, induced cell ...
<p>Lane M: protein MW markers; Lanes 1–2: IPTG induced <i>E. coli</i> pET30a-MANB; Lanes 3–5: Negati...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
<p>SDS-PAGE analysis of Mth10b expression and purification in <i>Escherichia coli</i>: lane 1, low m...
<p>A) SDS-PAGE. Lane 1: Precision Plus All Blue molecular weight standard (Bio-Rad). Lane 2: Soluble...
<p>SDS-PAGE analysis of the purified recombinant Xyl3A. Lanes: 1, the purified recombinant Xyl3A aft...
(Lane 1—Protein Standard (NEB, USA), Lane 2—Negative Control (E. coli BL21DE3), Lane 3—Uninduced clo...
<p>The purified proteins were separated on a 12.5% gel under reducing or non-reducing conditions: (A...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...
<p>Protein weight marker (lane M), cell extract of <i>E. coli</i> LMG194/pBADRSFP (lane A), pooled f...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<div><p><b>coli transformants</b>. </p> <p>Lane 1, <i>E</i>. <i>coli</i>/pET-MS-L-G; Lane 2, <i>E</i...
<p>Lane 1, Protein Molecular Weight Marker (Fermentas); lane 2, soluble <i>E. coli</i> BL21 (DE3) [p...
<p>M: molecular weight markers; 1: lysates of pre-IPTG-induced <i>E</i>. <i>coli</i> in 20 μL of cel...
<p>(A) Distribution of PD in induced bacteria after large-scale expression. Lane M, pre-stained prot...
<p>A) Lane M, protein molecular weight markers; lane 1, uninduced cell sample; line 2, induced cell ...
<p>Lane M: protein MW markers; Lanes 1–2: IPTG induced <i>E. coli</i> pET30a-MANB; Lanes 3–5: Negati...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
<p>SDS-PAGE analysis of Mth10b expression and purification in <i>Escherichia coli</i>: lane 1, low m...
<p>A) SDS-PAGE. Lane 1: Precision Plus All Blue molecular weight standard (Bio-Rad). Lane 2: Soluble...
<p>SDS-PAGE analysis of the purified recombinant Xyl3A. Lanes: 1, the purified recombinant Xyl3A aft...
(Lane 1—Protein Standard (NEB, USA), Lane 2—Negative Control (E. coli BL21DE3), Lane 3—Uninduced clo...
<p>The purified proteins were separated on a 12.5% gel under reducing or non-reducing conditions: (A...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...