SDS-PAGE and Western blot analysis of NC-SAPN (the basis of nanoparticle).

(A) SDS-PAGE analysis of NC-SAPN expression. Lanes 1–4: E. coli M15-pQE30, lanes 6–10: E. coli M15-NC-SAPN-pQE30. Lanes 1, 6: Before induction; lanes 2 and 7: 1h after induction with IPTG; lanes 3 and 8: 2h after induction; lanes 4 and 9: 4h after induction; lane 10: 16h after induction; and lane 5: molecular weight protein marker (Fermentas, 116–14.4 kDa). (B) SDS-PAGE analysis of purified NC-SAPN. Lanes 1–3: purified NC-SAPN. Lane 4: molecular weight protein marker (Fermentas, 116–14.4 kDa). (C) Western blot analysis of PfGCS1-SAPN protein with anti-His tag mAb. Lanes 1 and 2: Before induction and 4h after induction of E. coli M15-pQE30 as negative controls, respectively. Lanes 3 and 4: Before induction and 4h after induction of E. coli M15-NC-SAPN-pQE30, respectively. Lane 5: purified PfGCS1-SAPN.