<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; Lanes 2, precipitation of His-EDDIE-BnPRP1; Lane 1, <i>E</i>. <i>coli</i> BL 21 (DE3) cells transformed with pET30a plasmid as a control. Lane 3, purified His-EDDIE-BnPRP1. (B) Tricine-SDS-PAGE analysis of BnPRP1 renaturation. Lane M, protein molecular weight marker; Lanes 1, 2, refolded BnPRP1 after 8 h.</p
<p>Lane 1, protein standard; Lane 2, total proteins of <i>E. coli</i> transfected with pET28a-Es-FAB...
<p>SDS-PAGE (10%) of purified recombinant proteins from <i>E.coli</i> BL-21 transformed with pGEX-<i...
<p>M. Protein molecular weight marker; 1. Non-induced pET28a(+)/GmolGOBP1transformed BL21 (DE3) cell...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p>(A) SDS-PAGE of the whole <i>E. coli</i> cell extract after 3 h of induction of both constructs. ...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p>Lane-1: Non-induced pET32a (+)/McinOBP1 transformed BL21 (DE3) cells; 2: IPTG induced <i>E. coli<...
<p>Protein samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue. Lane 1,...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
<p>Lane 1, molecular mass standards. Lane 2, total protein of <i>E</i>. <i>coli</i> BL21 (DE3) pLysS...
<p>(A) SDS-PAGE of the products of the <i>E</i>. <i>coli</i> BL21(DE3)/pET22b-KT1 expression system....
(Lane 1—Protein Standard (NEB, USA), Lane 2—Negative Control (E. coli BL21DE3), Lane 3—Uninduced clo...
<p>(A) Restriction enzyme analysis of the recombinant plasmid pET-20b(+)-<i>pul</i>. Lane M: DNA Mar...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
<p>Lane 1, protein standard; Lane 2, total proteins of <i>E. coli</i> transfected with pET28a-Es-FAB...
<p>SDS-PAGE (10%) of purified recombinant proteins from <i>E.coli</i> BL-21 transformed with pGEX-<i...
<p>M. Protein molecular weight marker; 1. Non-induced pET28a(+)/GmolGOBP1transformed BL21 (DE3) cell...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p>(A) SDS-PAGE of the whole <i>E. coli</i> cell extract after 3 h of induction of both constructs. ...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p>Lane-1: Non-induced pET32a (+)/McinOBP1 transformed BL21 (DE3) cells; 2: IPTG induced <i>E. coli<...
<p>Protein samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue. Lane 1,...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
<p>Lane 1, molecular mass standards. Lane 2, total protein of <i>E</i>. <i>coli</i> BL21 (DE3) pLysS...
<p>(A) SDS-PAGE of the products of the <i>E</i>. <i>coli</i> BL21(DE3)/pET22b-KT1 expression system....
(Lane 1—Protein Standard (NEB, USA), Lane 2—Negative Control (E. coli BL21DE3), Lane 3—Uninduced clo...
<p>(A) Restriction enzyme analysis of the recombinant plasmid pET-20b(+)-<i>pul</i>. Lane M: DNA Mar...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
<p>Lane 1, protein standard; Lane 2, total proteins of <i>E. coli</i> transfected with pET28a-Es-FAB...
<p>SDS-PAGE (10%) of purified recombinant proteins from <i>E.coli</i> BL-21 transformed with pGEX-<i...
<p>M. Protein molecular weight marker; 1. Non-induced pET28a(+)/GmolGOBP1transformed BL21 (DE3) cell...