(Lane 1—Protein Standard (NEB, USA), Lane 2—Negative Control (E. coli BL21DE3), Lane 3—Uninduced clone, Lane 4—Induced clone, Lane 5—Purified peptidase).</p
<p>Lane 1: protein marker, Lane 2: purified Der p2. <b>Western blot analysis of recombinant Der p2-p...
<p>Lane M: protein molecular weight marker; Lane 1: total protein after degeneration; Lane 2: total ...
<p>Lane 1 showed whole-cell proteins of uninduced empty vector strain of <i>E</i>. <i>coli</i> BL21-...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; La...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
<p>Expression of BVDV-E2 in <i>E</i>. <i>coli</i> BL21 (DE3). When DE3 bacteria reached the logarith...
<p>Lane M: protein molecular weight marker; Lane 1: total protein of <i>E. coli</i> BL21(DE3); Lane ...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...
<p>Lane 1, molecular mass standards. Lane 2, total protein of <i>E</i>. <i>coli</i> BL21 (DE3) pLysS...
<p>Lane M: protein MW markers; Lanes 1–2: IPTG induced <i>E. coli</i> pET30a-MANB; Lanes 3–5: Negati...
<p>Lane M, marker; lane 1, the soluble fraction of the <i>E. coli</i> cell lysate containing TIM; la...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p>Lanes: M – MW markers, a - transformed <i>E. coli</i> BL21, b - transformed <i>E. coli</i> BL21 i...
<p>Lane 1: protein marker, Lane 2: purified Der p2. <b>Western blot analysis of recombinant Der p2-p...
<p>Lane M: protein molecular weight marker; Lane 1: total protein after degeneration; Lane 2: total ...
<p>Lane 1 showed whole-cell proteins of uninduced empty vector strain of <i>E</i>. <i>coli</i> BL21-...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; La...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
<p>Expression of BVDV-E2 in <i>E</i>. <i>coli</i> BL21 (DE3). When DE3 bacteria reached the logarith...
<p>Lane M: protein molecular weight marker; Lane 1: total protein of <i>E. coli</i> BL21(DE3); Lane ...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...
<p>Lane 1, molecular mass standards. Lane 2, total protein of <i>E</i>. <i>coli</i> BL21 (DE3) pLysS...
<p>Lane M: protein MW markers; Lanes 1–2: IPTG induced <i>E. coli</i> pET30a-MANB; Lanes 3–5: Negati...
<p>Lane M, marker; lane 1, the soluble fraction of the <i>E. coli</i> cell lysate containing TIM; la...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p>Lanes: M – MW markers, a - transformed <i>E. coli</i> BL21, b - transformed <i>E. coli</i> BL21 i...
<p>Lane 1: protein marker, Lane 2: purified Der p2. <b>Western blot analysis of recombinant Der p2-p...
<p>Lane M: protein molecular weight marker; Lane 1: total protein after degeneration; Lane 2: total ...
<p>Lane 1 showed whole-cell proteins of uninduced empty vector strain of <i>E</i>. <i>coli</i> BL21-...