<p>Lane M: protein molecular weight marker; Lane 1: total protein of <i>E. coli</i> BL21(DE3); Lane 2: total protein of <i>E. coli</i> BL21(DE3)/pET20b(+)-<i>pul</i>; Lane 3: total protein of <i>E. coli</i> BL21(DE3)/pET22b(+)-<i>pul</i>.</p
<p>Protein samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue. Lane 1,...
(Lane 1—Protein Standard (NEB, USA), Lane 2—Negative Control (E. coli BL21DE3), Lane 3—Uninduced clo...
<p>Lane M, protein molecular marker; Lane 1, before induction with 0.7 mM IPTG; Lane 2, after induct...
<p>Lane M: protein molecular weight marker; Lane 1: total protein after degeneration; Lane 2: total ...
<p>The PUL activities were compared between the two recombinants, <i>E. coli</i> BL21(DE3)/pET-20b(+...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p>(A) Restriction enzyme analysis of the recombinant plasmid pET-20b(+)-<i>pul</i>. Lane M: DNA Mar...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
<p><b>A</b> Lane M, protein molecular weight marker(low); Lane 1, the supernatant of the empty vecto...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p>Lane 1 showed the BL-PET30 as negative control; Lane 2~4 and lane 6~10 showed different recombina...
<p>M, protein marker; lanes 1 and 2, negative controls of untransformed vector and recombinant <i>E....
<p>Lane 1, molecular mass standards. Lane 2, total protein of <i>E</i>. <i>coli</i> BL21 (DE3) pLysS...
<p>Protein samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue. Lane 1,...
(Lane 1—Protein Standard (NEB, USA), Lane 2—Negative Control (E. coli BL21DE3), Lane 3—Uninduced clo...
<p>Lane M, protein molecular marker; Lane 1, before induction with 0.7 mM IPTG; Lane 2, after induct...
<p>Lane M: protein molecular weight marker; Lane 1: total protein after degeneration; Lane 2: total ...
<p>The PUL activities were compared between the two recombinants, <i>E. coli</i> BL21(DE3)/pET-20b(+...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p>(A) Restriction enzyme analysis of the recombinant plasmid pET-20b(+)-<i>pul</i>. Lane M: DNA Mar...
<p>Lane M, protein molecular weight markers; lane 1, the crude extract of the recombinant <i>E</i>. ...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
<p><b>A</b> Lane M, protein molecular weight marker(low); Lane 1, the supernatant of the empty vecto...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p>Lane 1 showed the BL-PET30 as negative control; Lane 2~4 and lane 6~10 showed different recombina...
<p>M, protein marker; lanes 1 and 2, negative controls of untransformed vector and recombinant <i>E....
<p>Lane 1, molecular mass standards. Lane 2, total protein of <i>E</i>. <i>coli</i> BL21 (DE3) pLysS...
<p>Protein samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue. Lane 1,...
(Lane 1—Protein Standard (NEB, USA), Lane 2—Negative Control (E. coli BL21DE3), Lane 3—Uninduced clo...
<p>Lane M, protein molecular marker; Lane 1, before induction with 0.7 mM IPTG; Lane 2, after induct...
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