<p>The PUL activities were compared between the two recombinants, <i>E. coli</i> BL21(DE3)/pET-20b(+)-<i>pul</i> (circle) and <i>E. coli</i> BL21(DE3)/pET-22b(+)-<i>pul</i> (square).</p
The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic chan...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
(A, B and C) Whole cell lysates prepared at 18 hours post infection from Vero cells infected at an M...
<p>Lane M: protein molecular weight marker; Lane 1: total protein of <i>E. coli</i> BL21(DE3); Lane ...
<p><i>E. coli</i> BL21(DE3)/pET-20b(+)-<i>pul</i> and <i>E. coli</i> BL21(DE3)/pET-22b(+)-<i>pul</i>...
(A) HeLa cells were transfected or co-transfected with the expression plasmid(s) indicated above eac...
<p>(A) Restriction enzyme analysis of the recombinant plasmid pET-20b(+)-<i>pul</i>. Lane M: DNA Mar...
<p>Lane M: protein molecular weight marker; Lane 1: total protein after degeneration; Lane 2: total ...
<p>Glycine was added in the auto-induction culture to the final concentration of 0 (star), 0.6% (cir...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
Currently, Escherichia coli (E. coli) is widely adopted as a host for recombinant genes to overexpre...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic chan...
The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic chan...
The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic chan...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
(A, B and C) Whole cell lysates prepared at 18 hours post infection from Vero cells infected at an M...
<p>Lane M: protein molecular weight marker; Lane 1: total protein of <i>E. coli</i> BL21(DE3); Lane ...
<p><i>E. coli</i> BL21(DE3)/pET-20b(+)-<i>pul</i> and <i>E. coli</i> BL21(DE3)/pET-22b(+)-<i>pul</i>...
(A) HeLa cells were transfected or co-transfected with the expression plasmid(s) indicated above eac...
<p>(A) Restriction enzyme analysis of the recombinant plasmid pET-20b(+)-<i>pul</i>. Lane M: DNA Mar...
<p>Lane M: protein molecular weight marker; Lane 1: total protein after degeneration; Lane 2: total ...
<p>Glycine was added in the auto-induction culture to the final concentration of 0 (star), 0.6% (cir...
<p>Lane M is protein molecular weight markers. Lane 1 is the crude extract of the recombinant strain...
Currently, Escherichia coli (E. coli) is widely adopted as a host for recombinant genes to overexpre...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic chan...
The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic chan...
The expression of interleukin -13 following induction with IPTG in E. coli results in metabolic chan...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
(A, B and C) Whole cell lysates prepared at 18 hours post infection from Vero cells infected at an M...
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