<p>M. Protein molecular weight marker; 1. Non-induced pET28a(+)/GmolGOBP1transformed BL21 (DE3) cells; 2. Induced pET28a(+)/GmolGOBP1transformed BL21 (DE3) cells; 3. Supernatant of disrupted pET28a(+)/GmolGOBP1 IPTG induced cells; 4. Inclusion body of disrupted pET28a(+)/GmolGOBP1 IPTG induced cells; 5. Purified rGmolGOBP1; 6. Non-induced pET28a (+)/GmolGOBP2 transformed BL21 (DE3) cells; 7. Induced pET28a(+)/GmolGOBP2 transformed BL21 (DE3) cells; 8. Supernatant of disrupted pET28a(+)/GmolGOBP2 IPTG induced cells; 9. Inclusion body of induced pET28a(+)/GmolGOBP2 IPTG induced cells; 10. Purified rGmolGOBP2.</p
<p>Lane 1 is HMW-GSs from common wheat variety Chinese Spring, the four expressed HMW-GSs are noted ...
<p>(A) SDS-PAGE of the whole <i>E. coli</i> cell extract after 3 h of induction of both constructs. ...
(A) SDS-PAGE analysis of PfGCS1-SAPN expression. Lanes 1–4: E. coli BL21(DE3)-pET24a, lanes 5–9: E. ...
<p>Lane-1: Non-induced pET32a (+)/McinOBP1 transformed BL21 (DE3) cells; 2: IPTG induced <i>E. coli<...
<p>Lane 1, protein standard; Lane 2, total proteins of <i>E. coli</i> transfected with pET28a-Es-FAB...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...
<div><p><b>coli transformants</b>. </p> <p>Lane 1, <i>E</i>. <i>coli</i>/pET-MS-L-G; Lane 2, <i>E</i...
<p>Protein samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue. Lane 1,...
<p><b>A</b> Lane M, protein molecular weight marker(low); Lane 1, the supernatant of the empty vecto...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p>M: protein molecular weight marker; 1: protein lysates from recombinant <i>E</i>. <i>coli</i> bef...
<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; La...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p>(A) The recombinant GmESR1 protein, induced with 0.5 mM IPTG at 37°C for 2, 4, 6, and 8 h in <i>E...
<p>Lane 1 is HMW-GSs from common wheat variety Chinese Spring, the four expressed HMW-GSs are noted ...
<p>(A) SDS-PAGE of the whole <i>E. coli</i> cell extract after 3 h of induction of both constructs. ...
(A) SDS-PAGE analysis of PfGCS1-SAPN expression. Lanes 1–4: E. coli BL21(DE3)-pET24a, lanes 5–9: E. ...
<p>Lane-1: Non-induced pET32a (+)/McinOBP1 transformed BL21 (DE3) cells; 2: IPTG induced <i>E. coli<...
<p>Lane 1, protein standard; Lane 2, total proteins of <i>E. coli</i> transfected with pET28a-Es-FAB...
<p><b>1</b>– Soluble fraction TcPR-10 mut; <b>2</b>– Insoluble fraction TcPR10 mut; <b>3</b>– Solubl...
<p><b>A</b>, expression time-courses of the fusion proteins in <i>E. coli</i>. Lane a, control 1, to...
<div><p><b>coli transformants</b>. </p> <p>Lane 1, <i>E</i>. <i>coli</i>/pET-MS-L-G; Lane 2, <i>E</i...
<p>Protein samples were separated by 15% SDS-PAGE and stained with Coomassie brilliant blue. Lane 1,...
<p><b>A</b> Lane M, protein molecular weight marker(low); Lane 1, the supernatant of the empty vecto...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p>M: protein molecular weight marker; 1: protein lysates from recombinant <i>E</i>. <i>coli</i> bef...
<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; La...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p>(A) The recombinant GmESR1 protein, induced with 0.5 mM IPTG at 37°C for 2, 4, 6, and 8 h in <i>E...
<p>Lane 1 is HMW-GSs from common wheat variety Chinese Spring, the four expressed HMW-GSs are noted ...
<p>(A) SDS-PAGE of the whole <i>E. coli</i> cell extract after 3 h of induction of both constructs. ...
(A) SDS-PAGE analysis of PfGCS1-SAPN expression. Lanes 1–4: E. coli BL21(DE3)-pET24a, lanes 5–9: E. ...