SDS-PAGE and Western blotting analysis of monomeric and polymer fractions in transgenic and control lines.

<p>A. SDS-PAGE analysis of reduced and unreduced fractions from flour of non-transgenic control (N-1) line, E-3 transgenic and E-5 transgenic lines. Lane M: Protein Marker. B. Western blotting analysis of reduced and unreduced fraction from flour of non-transgenic control (N-1) line, E-3 transgenic and E-5 transgenic lines. Lane M: Protein Marker. The black arrow (B) indicates the unreduced monomeric subunit, while the grey arrow (B) indicates the reduced form. The unreduced form of the subunit migrates faster probably due to its more compact conformation determined by the presence of intra-chain disulphide bonds.