SDS-PAGE and Western blot analysis of Atm.

<p>A. SDS-PAGE analysis. M: Protein markers; Lane 1: cell extract; Lane 2: unbound proteins in the flow through from the column; Lane 3: the fraction from the wash buffer containing 40 mM imidazole; Lane 4: the fraction from the wash buffer containing 100 mM imidazole; Lane 5: the fraction from the elution buffer containing 500 mM imidazole. B. Western blot analysis. M: Protein markers; lane 1: protein extracts from pG-KJE8/BL21 with pCold I—atm; lane 2: protein extracts from pG-KJE8/BL21 with pCold I, as a negative control; lane 3, protein extracts from pG-KJE8/BL21, as an additional negative control.