Western blot analysis of envelope glycoprotein incorporation in WT and mutant virus particles.

<p>Virus was concentrated, lysed and subjected to western blotting. Gp120, gp41 and p24 were detected in virus lysates of (A) mutants lacking a disulphide bridge and (B) <i>N</i>-glycosylation site mutants. (C-D) Quantification of protein levels was performed using the BioRad Image Lab Software, based on panels A and B. Gp160, gp120 and gp41 levels are presented after normalization to the p24 levels. Graphs represent the mean ± SEM based on 2–4 independent experiments. The difference between WT and mutant virus was considered to be significant when the <i>p</i> value calculated using the student’s t-test was <0.05 (* = p<0.05, for both gp120+gp160 and gp41).