The F-actin depolymerization co-regulated by ADF7-inhibited-VLN1 elevates ROS accumulation during root hair formation.

(A) Images of ROS levels in roots of Col-0, adf7-2, vln1-2, and adf7 vln1 #1 seedlings under Lat A treatments (0.2 μM) and CD (3 μM). Seedlings were treated with H2DCF–DA (see Methods), scale bar, 100 μm. (B) Histogram depicting relative fluorescence intensities of ROS accumulation in (A) At least 30 seedlings were examined for each material in different treatments. (C) Images of root hairs from Col-0, adf7-2, vln1-2, ADF7 OE #13, vln1-2, VLN1 OE #7, adf7 vln1 #1, and adf7 VLN1 OE #1 under 50 μM and 75 μM DPI treatments. A negative control was supplemented with 0.75% DMSO. Scale bar, 200 μm. (D) Histogram depicting root hair number in (C). (E) RT-qPCR quantification of RHD2 expression level in Col-0, adf7-2, vln1-2, and adf7 vln1 #1. Values are means ± SD of three independent biological replicates. *** P t-test compared to Col-0. Significant difference (P(B) and (D).