The F-actin depolymerization regulated by ADF7-inhibited-VLN1 promotes root hair formation.

(A) Images of root hairs from Col-0, adf7-2, ADF7 OE #14, vln1-2, VLN1 OE #8, and adf7 vln1 #1 under CK and Lat A treatments (0.2 μM). Scale bar, 200 μm. (B) Histogram depicting root hair number in (A). (C) Confocal microscopy images of epidermal cells visualized by the expression of fABD2::GFP in root apices from Col-0, adf7-2, vln1-2, and adf7 vln1 #1 seedlings under Lat A treatments (0.2 μM). Enlarged views from the red boxes are in the bottom row. Scale bar, 25 μm. (D) Violin plot showing the average and contribution of fluorescence intensity of actin cables in (C). The red line represents the average fluorescence intensity in different genotypes. (E) Histogram depicting skewness of F-actin in (C). (F) Histogram depicting the percentage of occupancy of F-actin in (C). Significant difference (P(B), (D), (E), and (F).