Low K-dependent ROS accumulation was obstructed in <i>ahk2ahk3</i> mutant and enhanced in <i>ipt1,3,5,7</i> mutant.

<p>(A) Pseudo-colored ROS fluorescence signals were detected in WT, <i>ahk2ahk3</i> and <i>ipt1,3,5,7</i> roots after staining with 20 µM of DFFDA for 20 min. Bar indicates 0.5 mm. (B) Quantification of DFFDA fluorescence signal shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047797#pone-0047797-g004" target="_blank">Figure 4A</a>. Pixel intensity of the roots was measured from the root hair differentiation zone to 0.5 mm. Different letters indicate significant differences from each other as determined using ANOVA (P<0.05) and significances were corrected post hoc using Tukey’s HSD comparisons. (n>20).