ADF7 inhibits the expression of function VLN1 in root hair formation.

(A) RT-qPCR quantification of VLN1 expression level in Col-0, adf7-2, ADF7 comp #2, ADF7 comp #7, ADF7 OE #13, and ADF7 OE #14. (B) RT-qPCR quantification of ADF7 expression level in Col-0, vln1-1, vln1-2, VLN1 comp #9, VLN1 comp #14, VLN1 OE #7, and VLN1 OE #8. (C) GUS analysis of VLN1 expression in root tips from Col-0, adf7-2, and ADF7 OE seedlings. (D) GUS analysis of ADF7 expression in root tips from Col-0, vln1, and VLN1 OE seedlings. (E) Western blotting of VLN1 expression level in Col-0, adf7-2, and ADF7 OE seedlings. Rubisco as a loading control. (F) Quantification of the relative grayscale value in (E). (G) Western blotting of ADF7 expression level in Col-0, vln1, and VLN1 OE seedlings. Rubisco as a loading control. (H) Quantification of the relative grayscale value in (G). (I) Images of root hairs from ADF7 and VLN1 double gene genotypes. Scale bar, 200 μm. (J) Histogram depicting root hair number in (I). Significant difference (PPt-test compared to Col-0, in (A) and (B), and compared to VLN1::GFP and ADF7::GFP in (F) and (H).