gDNA: Non converted genomic DNA (10 ng/reaction); pRef-LINE, pMe-LINE-1.1 and pUn-LINE-1.1: Linearized recombinant plasmids (4 pg equivalent to 106 copies); undetermined: Ct>36. Average CT values were calculated from 5 repeated reactions. (DOCX)</p
<p><b>Copyright information:</b></p><p>Taken from "Analysis of repetitive element DNA methylation by...
Primer design for PCR-based methylation analysis following bisulfite conversion of DNA is considerab...
Cot1 DNA. Two independent hybridizations were carried out using genomic DNA extracted from CD8lympho...
<p>A) Schematic illustration of the human <i>INS</i> gene promoter region showing the position of th...
<p><b>A–C</b>: gDNA (G) and cDNA (C) samples from a panel of human cell lines (described in <a href=...
<p>Standard curves were generated by qPCR using as template 5-fold serial dilutions of heteroplasmic...
(A) RT-PCR analysis of mgmt mRNA expression; gapdh served as a control. T1371 was studied during pas...
<p>Methylation-specific PCR (MSP) assay of three genes in MCF-7 cells (duplicate reactions for each ...
<p>(a-c) unmodified DNA primers and (d-f) 5′-<i>o</i>-TINA modified primers. Each ○ on the efficienc...
<p>(a) Melting curves of ten candidate reference genes and one target gene showing single peaks. (b)...
Background: DNA methylation is a well-studied epigenetic mark that is frequently altered in diseases...
1<p>Bold symbol represents the amplicons with good quality.</p>2<p>No. of CpG site = the number of C...
<p>(A) The position of primers. Dotted line shows genomic deletion (see <a href="http://www.plosone....
<p>(<b>A</b>) Comparison of specificity between specific primers and compound specific primers. Lane...
<p>The top figure used 100 nM primers for PCR and the bottom figure used 12.5 nM primers for PCR.</p
<p><b>Copyright information:</b></p><p>Taken from "Analysis of repetitive element DNA methylation by...
Primer design for PCR-based methylation analysis following bisulfite conversion of DNA is considerab...
Cot1 DNA. Two independent hybridizations were carried out using genomic DNA extracted from CD8lympho...
<p>A) Schematic illustration of the human <i>INS</i> gene promoter region showing the position of th...
<p><b>A–C</b>: gDNA (G) and cDNA (C) samples from a panel of human cell lines (described in <a href=...
<p>Standard curves were generated by qPCR using as template 5-fold serial dilutions of heteroplasmic...
(A) RT-PCR analysis of mgmt mRNA expression; gapdh served as a control. T1371 was studied during pas...
<p>Methylation-specific PCR (MSP) assay of three genes in MCF-7 cells (duplicate reactions for each ...
<p>(a-c) unmodified DNA primers and (d-f) 5′-<i>o</i>-TINA modified primers. Each ○ on the efficienc...
<p>(a) Melting curves of ten candidate reference genes and one target gene showing single peaks. (b)...
Background: DNA methylation is a well-studied epigenetic mark that is frequently altered in diseases...
1<p>Bold symbol represents the amplicons with good quality.</p>2<p>No. of CpG site = the number of C...
<p>(A) The position of primers. Dotted line shows genomic deletion (see <a href="http://www.plosone....
<p>(<b>A</b>) Comparison of specificity between specific primers and compound specific primers. Lane...
<p>The top figure used 100 nM primers for PCR and the bottom figure used 12.5 nM primers for PCR.</p
<p><b>Copyright information:</b></p><p>Taken from "Analysis of repetitive element DNA methylation by...
Primer design for PCR-based methylation analysis following bisulfite conversion of DNA is considerab...
Cot1 DNA. Two independent hybridizations were carried out using genomic DNA extracted from CD8lympho...