Genotyping PCR using flanking primer sets.

<p>(A) The position of primers. Dotted line shows genomic deletion (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006902#s3" target="_blank">results and discussion</a>). (B) PCR with the primers N008/N009 identifies either transgenic or non-transgenic mice, in which the product is detected as 400 bp band. On the other hand, multiple PCR with three primer sets clearly distinguish wild-type (single larger band), homozygous (single smaller band) and hemizygous (both bands) DNA. The expected size of PCR products are 500 bp and 295 bp with Chr18F1/Chr18R2/OUT1, and 1.5 kb and 1 kb with Chr18F5/R2/R3.