<p><b>(a)</b> Southern blot analysis of six (M1 to 6) heterozygous Flpe-excised KI mice. The genomic DNA of tested mice was compared to the DNA of a wild-type (WT) mouse. <i>Nhe</i>I-digested DNA was blotted onto a nylon membrane and hybridized with the external 3’ probe showing a 14.5-kb band for the WT allele and a 9.9-kb band for the Neo-excised allele (KI Muc5b-GFP) in agreement with the restriction map (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0174764#pone.0174764.g001" target="_blank">Fig 1a</a>). <b>(b)</b> Example of tail-DNA genotyping by PCR.</p
<p>The genotypes of viable mice at the indicated postnatal (P) or embryonic (E) day were determined ...
<div><p>a.The representative gel electropherogram of the genotyping result by BglII digestion of the...
Polymorphic genetic features, such as variable number of tandem repeats (VNTRs), are regarded as the...
<p>The genomic DNA extracted from six transgenic mice and one wild-type mouse was digested with EcoR...
<p>(A) Southern blot analysis of genomic DNA. SacI and BamHI digest of C3(1)Tag mouse genomic DNA (T...
<p><i>A. Targeting strategy for generating MLF1-IP KO mice</i> The red square on the 5′ side of the ...
<p>A) Construct and targeted genomic DNA before and after homologous recombination. The region of ex...
Polymerase chain reaction (PCR) based on single primers of arbitrary nucleotide sequence provides a ...
Method of mapping: Zfp15 was localized by haplotype analysis of 94 progeny from an interspecific bac...
Rat microsatellite primers were used for detection of homologous DNA segments in the mouse species (...
Twenty microsatellites were generated from a previously characterized lambda gt10 library containing...
In previous studies, genetically altered mice were produced which contained a transgene, providing a...
<p>(<b>A</b>) The normal wild-type (WT) allele of the <i>Btbd9</i> gene contains 12 exons. A commerc...
<p>(a). Schematic demonstrating the strategy for targeted integration of exogenous gene(s), wild typ...
Forty-three sequences containing simple sequence repeats or microsatellites were generated from an M...
<p>The genotypes of viable mice at the indicated postnatal (P) or embryonic (E) day were determined ...
<div><p>a.The representative gel electropherogram of the genotyping result by BglII digestion of the...
Polymorphic genetic features, such as variable number of tandem repeats (VNTRs), are regarded as the...
<p>The genomic DNA extracted from six transgenic mice and one wild-type mouse was digested with EcoR...
<p>(A) Southern blot analysis of genomic DNA. SacI and BamHI digest of C3(1)Tag mouse genomic DNA (T...
<p><i>A. Targeting strategy for generating MLF1-IP KO mice</i> The red square on the 5′ side of the ...
<p>A) Construct and targeted genomic DNA before and after homologous recombination. The region of ex...
Polymerase chain reaction (PCR) based on single primers of arbitrary nucleotide sequence provides a ...
Method of mapping: Zfp15 was localized by haplotype analysis of 94 progeny from an interspecific bac...
Rat microsatellite primers were used for detection of homologous DNA segments in the mouse species (...
Twenty microsatellites were generated from a previously characterized lambda gt10 library containing...
In previous studies, genetically altered mice were produced which contained a transgene, providing a...
<p>(<b>A</b>) The normal wild-type (WT) allele of the <i>Btbd9</i> gene contains 12 exons. A commerc...
<p>(a). Schematic demonstrating the strategy for targeted integration of exogenous gene(s), wild typ...
Forty-three sequences containing simple sequence repeats or microsatellites were generated from an M...
<p>The genotypes of viable mice at the indicated postnatal (P) or embryonic (E) day were determined ...
<div><p>a.The representative gel electropherogram of the genotyping result by BglII digestion of the...
Polymorphic genetic features, such as variable number of tandem repeats (VNTRs), are regarded as the...