Evaluation of MethyLight reactions toward the methylated and unmethylated versions of Alu, LINE-1, Satα and Sat2 sequences on a panel of DNA samples

<p><b>Copyright information:</b></p><p>Taken from "Analysis of repetitive element DNA methylation by MethyLight"</p><p>Nucleic Acids Research 2005;33(21):6823-6836.</p><p>Published online 2 Dec 2005</p><p>PMCID:PMC1301596.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Levels of methylation (in black) are expressed as PMR using DNA treated with M.I as a methylated reference. Levels of unmethylated DNA (in white) are expressed as PUR in which a WGA-DNA sample was used as an unmethylated reference. Each value represents the mean of 3–6 methylation measurements, except for the ALU-M3 reaction, which is the average of two measurements. Error bars indicate the standard error of the mean and have been omitted for the ALU-M3 reaction, as indicated by an asterisk, since we have only two PMR measurements for this reaction. We detected PMR or PUR values o