<p>Methylation-specific PCR (MSP) assay of three genes in MCF-7 cells (duplicate reactions for each gene). Primers for various genes in NT and PJ34-treated cells at corresponding CpG islands were used. Three biological replicates of samples were used.</p
<p>The methylation states of the DMRs of <i>Rasgrf1</i>, <i>Peg3</i>, and <i>Kcnq1ot1/Lit</i> are sh...
Heterogeneity of cells with respect to the DNA methylation status at a specific CpG site is a proble...
<p>MSP: methylation-specific polymerase chain reaction; M: methylation-specific primers; U: unmethyl...
Background: DNA methylation is a well-studied epigenetic mark that is frequently altered in diseases...
(A) RT-PCR analysis of mgmt mRNA expression; gapdh served as a control. T1371 was studied during pas...
<p>Representative 2% agarose gel electrophoresis images of PCR products for (A) ESR1 and (B) CCND2 g...
<p>M, markers; N, negative control (water blank); P, positive control (universal methylated DNA); T,...
In normal adult tissue, L gene promoters are generally unmethylated. In contrast, hypermethylation o...
<p>Primer sets utilized for PCR amplification are designated as unmethylated (U) or methylated (M), ...
Abstract. Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the...
A procedure for the analysis of the methylation status of imprinted genes is described. The method o...
ment of DNAmethylation, are important in the inves-tigation of syndromes influenced by imprinted gen...
<p>DNA was treated with sodium bisulfite and amplified with conversion-specific COBRA (combined bisu...
Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methy...
<p>A) Schematic illustration of the human <i>INS</i> gene promoter region showing the position of th...
<p>The methylation states of the DMRs of <i>Rasgrf1</i>, <i>Peg3</i>, and <i>Kcnq1ot1/Lit</i> are sh...
Heterogeneity of cells with respect to the DNA methylation status at a specific CpG site is a proble...
<p>MSP: methylation-specific polymerase chain reaction; M: methylation-specific primers; U: unmethyl...
Background: DNA methylation is a well-studied epigenetic mark that is frequently altered in diseases...
(A) RT-PCR analysis of mgmt mRNA expression; gapdh served as a control. T1371 was studied during pas...
<p>Representative 2% agarose gel electrophoresis images of PCR products for (A) ESR1 and (B) CCND2 g...
<p>M, markers; N, negative control (water blank); P, positive control (universal methylated DNA); T,...
In normal adult tissue, L gene promoters are generally unmethylated. In contrast, hypermethylation o...
<p>Primer sets utilized for PCR amplification are designated as unmethylated (U) or methylated (M), ...
Abstract. Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the...
A procedure for the analysis of the methylation status of imprinted genes is described. The method o...
ment of DNAmethylation, are important in the inves-tigation of syndromes influenced by imprinted gen...
<p>DNA was treated with sodium bisulfite and amplified with conversion-specific COBRA (combined bisu...
Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methy...
<p>A) Schematic illustration of the human <i>INS</i> gene promoter region showing the position of th...
<p>The methylation states of the DMRs of <i>Rasgrf1</i>, <i>Peg3</i>, and <i>Kcnq1ot1/Lit</i> are sh...
Heterogeneity of cells with respect to the DNA methylation status at a specific CpG site is a proble...
<p>MSP: methylation-specific polymerase chain reaction; M: methylation-specific primers; U: unmethyl...