Med16 deficiency primarily overactivates TFIID-dependent genes.

(A) Western blot showing the kinetics of Med16-AID depletion upon 3-IAA treatment of cells. (B) Spot assays assessing growth of the parental and Med16 strains on YPD plates containing DMSO or 500 μM 3-IAA at 37°C. (C) Volcano plots of nsRNA alterations in the med16Δ versus WT and Med16-AID 3-IAA versus DMSO comparisons. (D) Venn diagrams of overlap in genes significantly upregulated or downregulated in the Med16-AID and med16Δ cells. (E) Boxplots of log2 fold changes in nsRNA levels for differentially expressed (DE) genes in Med16-AID (left) and med16Δ (right) cells. Statistical differences between groups were assessed by Wilcoxon rank-sum test. (F) k-means clustered (k = 2) heatmap of log2 fold changes in nsRNA levels for the set of 932 genes with concordant changes in nsRNA levels in the Med16-AID and med16Δ strains. (G) Pie charts indicating the classification of Med16-regulated genes according to the categories of Donczew et al [31].